• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The present invention discloses a method based on micro-CT for determining the flow rate of assimilation products of crops from the family Rosaceae, comprising the following steps: Step 1: Establishing a micro-CT-based relationship formula between the sieve tube cross-sectional area (cross-sectional area of the vascular bundle) and the cross-sectional area of the stem of Rosaceae crops to determine the cross-sectional area of the vascular bundle of an internode of a Rosaceae crop to be measured; Step 2: Determine the concentration of assimilation products at both ends of the internode of the Rosaceae crop. Establishing a mannitol detection method based on near infrared spectroscopy and determining the mannitol concentration at both ends of the internode; Step 3: Calculate the flow rate of the assimilation products of the internode with the concentration field at both ends of the internode and the cross-sectional area of the vascular bundle. The present invention overcomes the deficiencies related to determining the flow rate of the assimilation products and provides a theoretical basis for the management of the stem shape and entire branches of Rosaceae crops; in this way a certain amount of support for the cultivation technique of the peach tree culture is achieved.
    公开号:CH714787B1
    申请号:CH01001/19
    申请日:2017-12-19
    公开日:2020-08-31
    发明作者:Ni Jiheng；Zhou Jingyu；Dong Jingtao；Mao Hanping
    申请人:Univ Jiangsu；
    IPC主号:
    专利说明:

    TECHNICAL AREA
    The present invention relates to the technical field of fruit tree cultivation, in particular a method based on micro-computed tomography (called “micro-CT” for short) for determining the flow rate of assimilation products of the cultivated plants from the Rosaceae family.
    STATE OF THE ART
    The cultivated plant from the Rosaceae family is a continuum of energy flow and material transport. The development of the cultivated plant requires the production of assimilation products by a "source", which are transferred to a "reservoir" via the transport organization in the form of an assimilation product flow. At the end a “yield” is formed in the “reservoir”.
    The accumulation process of the assimilation products in the “reservoir” is limited by the ability to provide the “source”, the efficiency of the reservoir and the transport capacity of the transport organization between the “source” and the “reservoir”. The transport capacity of the transport organization between the “source” and the “reservoir”, namely the flow capacity, is determined by the vascular bundle of the stem of the cultivated plant from the Rosaceae family, and the strength of the current and the area of the vascular bundle are strongly correlated. Current research focuses mainly on the regulation of the "sources" and the "reservoirs," while the exploration of the stream is less. Current methods of determining the rate of the assimilation product flow mainly include an aphid sting method and an isotope tracer method. The aphid sting method can damage the cultures and easily lead to diseases of the cultures, while the isotope tracer method can cause radioactive damage to the operator.
    CONTENT OF THE PRESENT INVENTION
    In view of the shortcomings of the prior art, the present invention provides a method for determining the assimilation product flow rate by examining the relationship between the assimilation product flow and the vascular bundle of the stems, and provides a theoretical basis for the establishment of the tree shape and the management of the Branches of a cultivated tree from the Rosaceae family.
    In order to achieve the above technical goal, the present invention employs the following technical solution:A method based on micro-CT for determining the flow rate of an assimilation product of a cultivated plant from the Rosaceae family, comprising the following steps: Step 1: Establishing a relationship formula between the sieve tube cross-sectional area and the cross-sectional area of the stem of the cultivated plant from the Rosaceae family based on by Micro-CT determination of the cross-sectional area of the sieve tubes of an internode of the cultivated plant from the Rosaceae family to be measured; Step 2: determining the concentration of the assimilation products of the two ends of the internode of the cultivated plant from the family Rosaceae with a hyperspectral imaging system; Step 3: Calculate the flow rate of the assimilation products of the internode with the concentration of the two ends of the internode of the cultivated plant from the family Rosaceae and the sieve tube cross-sectional area;
    The relationship formula between the sieve tube cross-sectional area and the cross-sectional area of the stem in step 1 is y = 8 × 10 <-5> x, where y is the sieve tube cross-sectional area and x is the cross-sectional area of the stem; and wherein the calculation formula is the cross-sectional area of a sieve tube, and wherein the sieve tube is regarded as a cylindrical body, and where d is the diameter of the sieve tube and m is the cross-sectional area of the sieve tube.
    Preferably, the two ends of the internode of the cultivated plant from the Rosaceae family are scanned with a hyperspectral imaging system in step 2 to obtain the reflection intensity of the sensitive wavelength of the assimilation products at both ends, the reflection intensity in a mannitol concentration prognostic modelC = K0 + K1 × X1 + K2 × X2 + K3 × X3 + K4 × X4 is introduced to calculate the mannitol concentration of both ends of the internode, and wherein in the formula, C is the mannitol concentration; and where K0, K1, K2, K3, K4 are spectral characteristics of a mannitol solution of different concentrations, while K0, K1, K2, K3, K4 are obtained by scanning with a hyperspectral imaging system and values 5.07, -1120, 52, 833, Have -640; and where X1, X2, X3, X4 are the reflection intensities of the sensitive wavelengths 452nm, 927.61nm, 1129.52nm, 1316.69nm, respectively; and wherein the mannitol concentration prediction modelC = 5.07 + (-1120) X1 + 52 X2 + 833 X3 + (- 640) X4ist.
    [0008] The solutions of different mannitol concentrations are preferably prepared in such a way that different mannitol masses are dissolved in distilled water in volumetric flasks, which are then made up quantitatively in order to obtain the specific different mannitol concentrations.
    It is preferably assumed in step 3 that the measured concentration of the assimilation products at the top of an internode of the Rosaceae culture C1, the concentration of the assimilation products at the bottom C2 and the measured time is T1 and T2, respectively, the amount of in the time period (T2-T1) assimilation products passing through the internode is (C1 × S1-C2 × S2), and where S1 and S2 are the sieve tube cross-sectional areas at the top and at the bottom, respectively; and wherein the internode has a flow rate of assimilation products of (C1 × S1 - C2 × S2) / (T2-T1) in the period from T1 to T2.
    The present invention has the following advantages: The current methods for determining the flow rate of the assimilation products mainly include an aphid sting method and an isotope tracer method. With the two methods, the flow rate of the assimilation products of the cultivated plant belonging to the Rosaceae family can be well determined, but there are defects that diseases of the cultures and harm to the operators are easily caused. The method for determining the flow rate of assimilation products of the cultivated plant from the family of Rosaceae cultures according to the present invention overcomes the shortcomings of the current determination methods and measures relatively well the flow rate of assimilation products of the cultivated plant from the family Rosaceae, and provides a theoretical basis for the Tree shape and the management of the crown size of the cultivated plant from the Rosaceae family.
    BRIEF DESCRIPTION OF THE DRAWING
    [0011] FIG. 1 shows a CT diagram of the scanning of the sieve tubes of a third internode of a peach tree.
    DETAILED DESCRIPTION
    The technical solution of the present invention is explained in more detail below, but the scope of protection of the present invention is not limited thereto.
    A method based on micro-CT for determining the flow rate of assimilation products of the cultivated plant from the family Rosaceae is carried out with an example of the peach tree in the following steps:First, an image of the stem of the peach tree is obtained by micro-CT. The diameter of the sieve tubes is determined by the picture. The cross-sectional area of the sieve tubes is calculated based on the diameter. Then the diameter of the internode is measured with a caliper to calculate the cross-sectional area of the internode. A mathematical formula is then established between the sieve tube cross-sectional area and the cross-sectional area of the internode. At the end, the mannitol concentrations in the vascular bundles at both ends of the internode of the stem are measured using near-infrared spectroscopy. The flow rate of assimilation products is calculated on the basis of the difference in the mannitol concentrations in the vessel bundles at both ends.
    The concrete steps are as follows:
    Step 1: Determination of the sieve tube cross-sectional area of the internode: (1) Establishing a micro-CT-based relationship formula between the sieve tube cross-sectional area and the cross-sectional area of the stem of the peach tree, namely: 1) Scanning an internode of the stem of the peach tree to obtain a CT To obtain the image of the internode of the peach tree, find out the sieve tubes of the stem of the peach tree on the CT image, then the number and diameter of the sieve tubes of the internode of the peach tree can be obtained, using the scale on the CT image will be the sieve tube cross-sectional area of the internode calculated; 2) The diameter of the internode is measured with the vernier caliper, then the cross-sectional area of the internode, namely the cross-sectional area of the stem, is calculated; 3) With the ratio between the sieve tube cross-sectional area and the cross-sectional area of the internode, a relationship formula is established between the two; (2) Determination of the measured sieve tube cross-sectional area of the internode, namely:The cross-sectional area of the internode is measured with the vernier caliper, the sieve-tube cross-sectional area of the internode can be obtained directly using formula (1).
    Step 2: Determining the concentration of assimilation products at both ends of the internode of the peach tree:With regard to the peach tree, the flow of the assimilation product in the peach tree is in the form of mannitol flow. With a hyperspectral imaging system (near infrared and visible light imaging system) the top and bottom ends, or top and bottom, of an internode are scanned in order to obtain the reflection intensity of the mannitol-sensitive wavelengths at both ends of the internode. Then the reflection intensity is entered into the mannitol concentration forecasting model to calculate the mannitol concentration at both ends of the internode.
    Step 3: Calculate the flow rate of assimilation products of the internode of the peach tree:It is assumed that the measured mannitol concentration at the top of an internode of the peach tree is C1, the mannitol concentration at the bottom is C2, and the measured time is T1 and T2, respectively, the amount of which is in the period (T2-T1) by the internode through assimilation products is (C1 × S1-C2 × S2), and where S1 and S2 are the cross-sectional area of the sieve tube at the top and bottom, respectively (obtained by measuring the diameter of the internode of the stem at both ends and then the Step 1 is performed); and wherein the internode has a flow rate of assimilation products of (C1 × S1-C2 × S2) / (T2-T1) in the period from T1 to T2.
    Detailed embodiment:
    Step 1: Establishing a micro-CT based relationship formula between the sieve tube cross-sectional area and the cross-sectional area of the stem of the peach tree:
    (1) selecting a normally growing peach tree plant and placing the peach tree plant in a micro-CT measuring cylinder for scanning, the scan site being the third internodal limb of the peach tree;Analyze the scanned CT image, specifically as follows:
    Finding the number and diameters of the sieve tubes in the image and calculating the cross-sectional area of the sieve tubes of the stem;FIG. 1 shows a sieve tube of the third link, with the scale in FIG. 1 the diameter of the sieve tubes is determined. As shown in Figure 1, the diameters of the sieve tubes are respectively d1-d8, the sieve tubes are regarded as cylindrical bodies, with the formula where m is the cross-sectional area of a sieve tube, the cross-sectional area of the sieve tubes of m1, m2-m8 is obtained; In Figure 1, the number of sieve tubes is 8, the diameter of each sieve tube is 14 µm, 16 µm, 26 µm, 23 µm, 28 µm, 14 µm, 17 µm, 15 µm, then the cross-sectional area of each sieve tube is 153.86 µm 2, 200.96 µm 2 , 530.66µm <2>, 415.27µm <2>, 615.44µm <2>, 153.86µm <2>, 226.87µm <2>, 176.63µm <2>, and the sieve tube cross section is 2473.55µm <2>; a caliper is used to measure the diameter of the third link, which is 3000 µm, the cross-sectional area is 28260000 µm <2>; With the ratio between the cross-sectional area of the sieve tube and the cross-sectional area of the internode, the relationship formula between the cross-sectional area of the stem and the cross-sectional area of the sieve tube is obtained as follows:y = 8 × 10 <-5> x (1)In the formula (1), y is the sieve tube cross-sectional area and x is the cross-sectional area of the stem, and the unit of them is µm 2;
    Step 2: Measuring the mannitol concentration at both ends of the third internode: (1) Establishing the mannitol concentration model:Weighing different amounts of mannitol of 5g, 4g, 3g, 2g and 1g (analytical purity) and dissolving each in distilled water in a 100 ml volumetric flask, then filling this up to make up solutions with respective mannitol concentrations of 50%, 40%, Get 30%, 20% and 10%; Sucking 1ml of each prepared mannitol solution and adding dropwise each sucked mannitol solution into a Petri dish; Flattening and air-drying the drops of each mannitol solution; and scanning the petri dish containing the air-dried drop with a hyperspectral imaging system (near infrared and visible light spectroscope) to obtain the spectral properties of the solutions of different mannitol concentrations and to pull the spectral characteristics of the solutions of different concentrations. The corresponding equations between the spectral parameters and the mannitol concentrations are set up as follows:C = K0 + K1 × X1 + K2 × X2 + K3 × X3 + K4 × X4 (2)In the formula (2), C is the concentration of mannitol; Ko, K1K2, K3, K4 are spectral characteristic values whose values from the spectral properties are 5.07, -1120, 52, 833, -640; and X1, X2, X3, X4, the values of which are empirically obtained by the hyperspectral imaging system, are the reflection intensities at the wavelengths 452nm, 927.61nm, 1129.52nm, 1316.69nm, each sensitive to a different concentration of mannitol.Then the mannitol concentration forecasting model is as follows:C = 5.07 + (-1120) X1 + 52 X2 + 833 X3 + (- 640) X4 (3) (2) Measure the mannitol concentration at both ends of the internode of the peach tree:The top and bottom of an internode of the peach tree are scanned with a hyperspectral imaging system (near infrared and visible light spectroscope). Then the spectral properties at both ends are analyzed. The reflection intensities X1, X2, X3, X4 at the sensitive wavelengths 452nm, 927.61nm, 1129.52nm, 1316.69nm are measured. Then the reflection intensities X1, X2, X3, X4 at the four sensitive wavelengths are introduced into the formula (3) to obtain the mannitol concentrations at the top and bottom of the third internode. At the same time, the measurement time is recorded.
    Step 3: Calculate the flow rate of the assimilation products: The measured concentration of mannitol at the top of an internode of the peach tree is 30g / l, and the concentration of mannitol at the bottom is 40g / l. The measured time is 10:00 and 11:00 respectively. The cross-sectional area of the sieve tube at the top and the bottom is 2600 µm <2> and 3000 µm <2>, namely in the period (10: 00-11: 00) the flow rate of the assimilation products of the internode in the period from T1 to T2 is 0.048 g / hour. The present invention has been briefly explained above and is not limited to this scope. The simple changes made based on the spirit and working method of the present invention and applied to other systems, or improvements and modifications without changing the main concept of the present invention, are to be considered included within the scope of the present invention, respectively.
    权利要求:
    Claims (7)
    [1]
    1. Method based on micro-CT for determining the flow rate of an assimilation product flow of a cultivated plant from the family Rosaceae, characterized in that it comprises the following steps:Step 1: Establishing a relational formula between the sieve tube cross-sectional area and the cross-sectional area of the stem of the rosaceae family crop based on micro-CT, and finding the sieve tube cross-sectional area of an internode of said Rosaceae family crop;Step 2: determining the concentration of the assimilation product of the two ends of the internode of said crop with a hyperspectral imaging system;Step 3: Calculate the flow rate of the assimilation product stream of the internode from the concentrations of the assimilation product at the upper and lower ends of the internode of the rosaceae family crop and the sieve tube cross-sectional area.
    [2]
    2. Method based on micro-CT for determining the flow rate of an assimilation product stream of the cultivated plant from the family Rosaceae according to claim 1, characterized in that the relationship formula between the sieve tube cross-sectional area and the cross-sectional area of the stem in step 1 y = 8 × 10 <-5 > x, where y is the sieve tube cross-sectional area and x is the cross-sectional area of the stem.
    [3]
    3. Method based on micro-CT for determining the flow rate of an assimilation product stream of a cultivated plant from the family Rosaceae according to claim 1, characterized in that the calculation formula of the cross-sectional area of a sieve tube is where the sieve tube is regarded as a cylindrical body, and where d is the diameter of the sieve tube and m is the cross-sectional area of the sieve tube.
    [4]
    4. Method based on micro-CT for determining the flow rate of an assimilation product stream of the cultivated plant from the family Rosaceae according to claim 1, characterized in that in step 2 the two ends of the internode of said cultivated plant are scanned with a hyperspectral imaging system in step 2, to measure the reflection intensities at the sensitive wavelengths, each sensitive to a different mannitol concentration, of the assimilation product at both ends of the internode, the values of the reflection intensities being introduced into a mannitol concentration prediction model in order to determine the mannitol concentrations of the calculate both ends of the internode.
    [5]
    5. Method based on micro-CT for determining the flow rate of an assimilation product of the cultivated plant from the family Rosaceae according to claim 4, characterized in that the mannitol concentration forecasting model C = K0 + K1 × X1 + K2 × X2 + K3 × X3 + K4 x X4, where in the formula C is the concentration of mannitol; where K0, K1, K2, K3, K4 are spectral characteristics of solutions of different mannitol concentrations, which have been obtained by scanning with a hyperspectral imaging system and have values 5.07, -1120, 52, 833, -640; and where X1, X2, X3, X4 are the reflection intensities of the sensitive wavelengths 452, 927.61, 1129.52, 1316.69 nm, respectively.
    [6]
    6. Method based on micro-CT for determining the flow rate of an assimilation product of the cultivated plant from the family Rosaceae according to claim 5, characterized in that the solutions of different mannitol concentrations are prepared in such a way that different amounts of mannitol are dissolved in distilled water in volumetric flasks, which are then quantitatively replenished to obtain the solutions of specific different concentrations of mannitol.
    [7]
    7. A method based on micro-CT for determining the flow rate of an assimilation product of the cultivated plant from the family Rosaceae according to claim 1, characterized in that, in step 3, the measured concentration of the assimilation product at the upper end of the internode of the cultivated plant at a time T1 is C1; that the concentration of the assimilation product at the lower end of the internode of the crop and a time is T2, C2; that the amount of assimilation product that passes through the internode during the period (T2-T1) is (C1 × S1-C2 × S2), where S1 and S2 are the sieve tube cross-sectional areas at the top and bottom of the internode, respectively; and wherein the assimilation product in the internode has a flow rate of (C1 × S1-C2 × S2) / (T2-T1) during the period from T1 to T2.
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    同族专利:
    公开号 | 公开日
    CN107942005A|2018-04-20|
    WO2019113997A1|2019-06-20|
    引用文献:
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    法律状态:
    2020-09-15| PFUS| Merger|Owner name: JIANGSU UNIVERSITY, CN Free format text: FORMER OWNER: JIANGSU UNIVERSITY, CN |
    优先权:
    申请号 | 申请日 | 专利标题
    CN201711338139.7A|CN107942005A|2017-12-14|2017-12-14|The assay method of rose family crop assimilation products flow velocity based on Micro CT|
    PCT/CN2017/117153|WO2019113997A1|2017-12-14|2017-12-19|Micro-ct-based method for measuring flow velocity of assimilation products of rosaceae crop|
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